Bioactive Analysis of Auricularia Delicata: Extraction, Purification, and Characterization of Polysaccharides from Auricularia Delicata-Morocco Forest

Abstract

Auricularia mushrooms are bioactive compounds which are mostly polysaccharides. Many studies have shown the use of Auricularia from wild mushroom for medicinal purposes such as antioxidant, antidiabetic, antitumor, hyperlipidemic, immunomodulatory, hepatoprotective and anticoagulant effect. Despite the fact that some studies showed pharmacological functions of polysaccharides extracted from Auricularia Delicata, the efficiency and the chemical process of extraction processes of extraction of polysaccharides from Auricularia were not clear. Therefore, this study proposes a method to purify and characterize the polysaccharide extracted from Auricularia through a method in Yoon et al. (2003). Polysaccharides characterization was done by colorimetric (Phenol-Sulfuric acid method). UV–Vis spectrophotometer was used to take colorimetric analysis of all solutions. The absorbance of the characteristic yellow-orange color was measured at 490 nm for hexose monosaccharide and 480 nm for pentose monosaccharide and uronic acid. The yield of purified polysaccharides from Auricularia was 0.88% with 80.4% purity expressed as glucose/xylose. We successfully obtained the purified polysaccharides from Auricularia with high purity.